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ISHS Acta Horticulturae 597: International Conference on Medicinal and Aromatic Plants (Part II)

COMPARATIVE STUDY OF THE VOLATILE CONSTITUENTS FROM JUNIPERUS COMMUNIS BERRIES OBTAINED UNDER DIFFERENT METHODS OF EXTRACTION

Authors:   P.S. Chatzopoulou, S.T. Katsiotis
Keywords:   GC/MS analysis, hydro distillation, headspace, Juniper berries, supercritical fluid extraction, volatile constituents
Abstract:
The volatile constituents obtained from Juniperus communis berries by the application of different methods, Hydro distillation (HD), Super Critical Fluid Extraction (SFE) and Headspace (HSE) static technique were examined as regards their qualitative and quantitative composition. Significant differences were noticed among the percentages of the main components of the extracts: the monoterpene hydrocarbons, the oxygenated ones and the sesquiterpenoids. Monoterpene hydrocarbons predominated using all three methods. The highest yield - as far it concerns the main constituents (89.9%) was obtained using the headspace technique, following by 60.6% in the distilled oil and 56.2% in the supercritical CO2 extract. α-Pinene was the most abundant monoterpene hydrocarbon, ranging from 51.3% to 36.4%, in the headspace and SFE-aroma products, respectively. The percentages of the sesquiterpene constituents varied also among the extracts. The SFE extraction was more effective giving 22.7% versus 21.9% in the hydro distilled oil, while the equilibrium headspace technique gave only 4.2%.

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