ISHS Acta Horticulturae 597: International Conference on Medicinal and Aromatic Plants (Part II)
IN VITRO VOLATILE OIL PRODUCTS OF MELALEUCA ERICIFOLIA SMITH |
| Author: | E.M.A. Youssef |
| Keywords: | In vitro, volatile oil, Melaleuca ericifolia, Gamma -ray, colchicine |
| Abstract:
The effect of gamma–irradiation and colchicine treatments on the yield and spectrum of volatile oil products of Melaleuca ericifolia explants in vitro were studied. Using a low dose of gamma-rays (10 Gy) resulted in the highest yield of volatile oil. Applying a high concentration of colchicine (400 ppm) or a low dose of gamma-rays (10 Gy) remarkably maximized the methyl eugenol content. Colchicine treatment at 200 ppm exhibited the maximum relative amounts of thujone, limonene and terpinyl acetate. In the case of a high dose of gamma-rays (40 Gy), the highest levels of p-cymene and  -terpineol were determined.
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