ISHS Acta Horticulturae 597: International Conference on Medicinal and Aromatic Plants (Part II)
EFFECT OF BIOTIC STRESS (ASPERGILLUS NIGER) ON THE PRODUCTION AND ACCUMULATION OF TOTAL ALKALOIDS IN ATROPA BELLADONNA L. VIA TISSUE CULTURE |
| Author: | H.S. Taha |
| Keywords: | Aspergillus niger, Atropa belladonna, biotic stress |
| Abstract:
An efficient protocol for enhancement of total alkaloids production from suspension cultures of A. belladonna L. was established. The effect of MS-medium supplemented with different concentrations of naphthalene acetic acid (NAA) and benzyl adenine (BA) on growth parameters as well as total alkaloid production was investigated. In the same connection, the effect of biotic stress caused by various concentrations of Aspergillus niger on alkaloid accumulation and production was investigated. The optimum values of cell growth parameters and alkaloid production were obtained for leaf, stem and root cell cultures, respectively. The optimum supplementation of liquid MS-medium was 1 mg/l of each of NAA and BA. A. niger extract at 10 % (~ 0.5 mg/ mL) gave the highest value for cell growth and alkaloid accumulation in the different types of cell cultures following 10 days of cultivation. Total alkaloid contents of different cell cultures were identified by HPLC. | |
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