ISHS Acta Horticulturae 596: VIII International Symposium on Pear
ELIMINATION OF APPLE CHLOROTIC LEAF SPOT VIRUS (ACLSV) FROM PEAR BY IN VITRO THERMOTHERAPY AND CHEMOTHERAPY |
| Author: | M. Cieslinska |
| Keywords: | pear ring pattern mosaic, ribavirin, Virazole, phytotoxicity, shoots survival, heat therapy |
| Abstract:
Micropropagated shoots of ‘Pierre Corneille’ pear plants infected with Apple chlorotic leaf spot virus (ACLSV) were subjected to heat therapy at 36 oC or/and chemotherapy using ribavirin (Virazole) to eliminate the virus. The presence of virus in recovered plants was evaluated by ELISA technique during one year after therapy. The majority of treated pear shoots survived thermotherapy. The effect of chemotherapy depended on the concentration of Virazole. Shoots treated with the 50 and 100 mg/L of Virazole showed chlorosis and apex necrosis. The highest concentration of this compound (100 mg/L) appeared to be highly phytotoxic for 77% of pear shoots. Almost all shoots of pear treated with high temperature and 100 mg/L Virazole died. Thermotherapy of pear ‘Pierre Corneille’ shoots enabled to eradicate ACLSV from 22% of plantlets. ELISA did not show any presence of ACLSV in 78 and 88% of pear shoots treated with Virazole in concentrations 25 and 50 mg/L respectively. The combination of thermotherapy and chemotherapy gave no better results than chemotherapy alone. | |
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