ISHS Acta Horticulturae 596: VIII International Symposium on Pear
VASCULAR TISSUE REGENERATION IN ”IN VITRO” GRAFTS OF PEAR/QUINCE |
| Authors: | L. Espen, M. Cocucci, G.A. Sacchi |
| Keywords: | graft incompatibility, vascular tissue regeneration, programmed cell death, Pyrus communis, Cydonia oblonga |
| Abstract:
In vitro grafting of shoot internodes excised from pear (Pyrus communis, cv Kaiser and cv Butirra Hardy), and quince (Cydonia oblonga, East Malling clone C) in vitro cultured plants well mimics, in short time, the histological and functional responses of (in)compatibility exhibited by the respective wooden plants. In the incompatible Kaiser/EM clone C (K/EM.C) combination an evident delay in the cohesion of the graft partners and, over all, a poor differentiation of functional wound-phloem connections were observed with respect to the compatible Butirra Hardy/EM clone C (BH/EM.C) ones. In the compatible combination, but not in the incompatible ones, the onset of the programmed cell death (PCD) was detectable in the graft region. The results indicate that in vitro grafting of shoot internodes is a suitable technique to study the localised type of graft incompatibility. Moreover, they suggest that K/EM.C incompatibility might be related to some effect of the graft union on the active program of PCD and in turn on the processes driving to differentiation of vascular bridges in the graft region. | |
Download Adobe Acrobat Reader (free software to read PDF files) | |
