ISHS Acta Horticulturae 596: VIII International Symposium on Pear
MICROPROPAGATION OF PEAR THROUGH TEMPORARY IMMERSION |
| Authors: | C. Damiano, A. Frattarelli, M. Giorgioni |
| Keywords: | propagation, biotechnology, in vitro, Pyrus communis, Pyrus communis var. pyraster |
| Abstract:
A procedure for the mass propagation of wild pear (Pyrus communis var pyraster L.) and pear (P. communis, cultivars Williams, Precoce di Fiorano, Conference and Harvest Queen) using a temporary immersion technique is described. The plant material consisted of in vitro shoots grown in LP medium (Quoirin et al., 1977) supplemented with BAP 0.4 mgl-1 and IBA 0.05 mgl-1. Three culture methods have been compared: solid, liquid and temporary immersion (for 30 and 60 minutes). In the case of wild pear the best results have been obtained with an immersion of 30 minutes (multiplication rate 13.3 per shoot). In the other cultivars the results changed according to the genotype: for ‘Conference’ an immersion of 30 minutes per day has provided the best multiplication rate, on the contrary for cultivars ‘Williams’ and ‘Harvest Queen’ an immersion of 60 minutes has been the best condition. Finally, in ‘Precoce di Fiorano’ the best growth was obtained with solid medium. Stationary liquid medium caused a high hyperhydricity: only ‘Harvest Queen’ has shown a good growth. In the wild pear and pear the temporary immersion technique revealed to be really efficient to prevent necrosis of the apices. Rooting in temporary immersion has been obtained successfully in all the cultivars of pear used, as well as in the wild pear. | |
Download Adobe Acrobat Reader (free software to read PDF files) | |
