ISHS Acta Horticulturae 596: VIII International Symposium on Pear
PEAR TRANSFORMATION WITH THE GENE FOR SUPERSWEET PROTEIN THAUMATIN II |
| Authors: | V.G. Lebedev, S.A. Taran, V.V. Shmatchenko, S.V. Dolgov |
| Keywords: | Pyrus communis, agrobacterial transformation, resistance to phytopathogene, taste modification |
| Abstract:
The gene for supersweet protein thaumatin II from Thaumatococcus danielli has been transferred to pear in attempts to improve taste. Based on the coding sequence, cloned at Unilever, and pBI121, we constructed the binary vector for its expression in plants. The successful transformation of the pear cultivar Burakovka has been obtained by using the disarmed supervirulent agrobacterial strain CBE21. Leaves from transgenic lines demonstrated taste modification, detectable by organoleptical test. The introduction of thaumatin gene was confirmed by PCR analysis and its expression by Western blotting. | |
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