ISHS Acta Horticulturae 596: VIII International Symposium on Pear
TRANSGENIC PEAR CLONAL ROOTSTOCKS RESISTANT TO HERBICIDE "BASTA" |
| Authors: | V.G. Lebedev, S.V. Dolgov, K.G. Skryabin |
| Keywords: | Pyrus communis, agrobacterial transformation, regeneration, phosphinotricin, kanamycin |
| Abstract:
Herbicides are widely used in nurseries and orchards. Herbicide-resistant rootstock for fruit production is a new way to confer selectivity and enhance fruit crop safety and production. The bar gene cloned in “Bioengineering Center” RAS has been used in our research for obtaining phosphinotricine (PPT)-resistant pear clonal rootstocks. This gene encodes a phosphinotricine acetyl transferase (PAT) which converts PPT into the non-toxic acetylated form and prevents the toxicity accumulation in plant tissues. Nontransformed and transgenic lines with nptII and gus genes of clonal pear rootstock GP217 were used in our research. Based on development transformation protocols, 17 transgenic PPT-resistant clones were obtained. The transformation frequency was 0.9-5.1%. PPT was inefficient as a selective agent because of the high frequency of escapes. Most transgenic lines without damage proliferated on medium with 250 mg/L PPT, control plants died on 2 mg/L. The bar integration into plant genomes was confirmed by PCR analysis. The transgenic plants expressing various levels of PAT were resistant for commercial herbicide “Basta”. | |
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