ISHS Acta Horticulturae 596: VIII International Symposium on Pear
DISCRIMINATION OF PEAR CULTIVARS WITH RAPD, AFLPTM AND ISSR |
| Authors: | L. Monte-Corvo, L. Goulão, C. Oliveira |
| Keywords: | Pyrus communis, cultivar identification, genetic relationship |
| Abstract:
In this work the results previously reported from random amplified polymorphic DNA (RAPD), amplified fragment length polymorphism (AFLPTM) and inter-simple sequence repeated (ISSR) techniques were used to compare their capacity to discriminate cultivars and to evaluate the genetic diversity among 24 cultivars of Pyrus communis. The three marker systems studied did not show relevant differences in polymorphism detection (informativeness) nor in genetic similarity estimates among cultivars. However, they presented considerable divergences in the number of markers generated per primer (Multiplex Index), which influenced the differences observed in efficiency of the marker systems. AFLP obtained the highest Marker Index value (11.73), followed by ISSR (8.81), while RAPD presented the lowest (2.06). Nevertheless ISSR markers were technically simpler, faster and cheaper than AFLP and they appeared to be the best technique for large-scale DNA fingerprinting analysis, for genetic diversity studies and germplasm evaluation. In spite of low correlation values of genetic similarities calculated between marker systems, the ISSR and AFLP dendrograms showed similar cultivars cluster. A more consistent and robust dendrogram was constructed, combining the results from the three marker systems, and could confirm or reinforce the results previously reported with each marker alone. | |
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