ISHS Acta Horticulturae 596: VIII International Symposium on Pear
FIRST DOUBLED HAPLOID PLANTS OF PEAR (PYRUS COMMUNIS) |
| Authors: | L. Bouvier, P. Guerif, M. Djulbic, Y. Lespinasse |
| Keywords: | pear, doubled haploid, oryzalin |
| Abstract:
Perennial fruit trees such pear require a long-term effort for breeding because of their long generation time and high level of heterozygosity. The production of haploid plants should offer new possibilities for genetic studies and breeding. Procedures for the production of haploid plants from peat have been developed. Oryzalin which was an efficient mitotic agent for doubling chromosomes of haploid apples shoots in vitro was tried in vitro on haploid pear. Clones from three cultivars of pear were tested and all were successfully doubled. The best concentrations of oryzalin were 200µM to 300µM but it was clone dependent. In vitro grafting of doubled shoots were carrying on ‘Old Home’ shoots rooted in vitro and acclimatized in greenhouse. | |
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