ISHS Acta Horticulturae 596: VIII International Symposium on Pear
S-ALLELE CHARACTERIZATION IN SELF-INCOMPATIBLE PEAR (PYRUS COMMUNIS): BIOCHEMICAL, MOLECULAR AND FIELD ANALYSES |
| Authors: | S. Zuccherelli, W. Broothaerts, P. Tassinari, S. Tartarini, L. Dondini, A. Bester, S. Sansavini |
| Abstract:
Gametophytic self-incompatibility (GI) is a natural mechanism in flowering plants, including pear and other fruit tree species, to prevent inbreeding and promote outcrossing. It is usually under control of one locus with more allelic variants (S1, S2, ..., Sn). The present study employed biochemical (isoelectric focusing, IEF) and molecular (S-PCR) methods to determine the S-allele composition of ten European pear cultivars,thereby obviating time-consuming field work whose results are often ambiguous because of environmental effects. The S locus composition of the ten cultivars was determined via S-PCR. To verify the S-PCR assay, two putative S-allele DNA fragments of Japanese pear were isolated and their relative sequences proved to be identical to the ones already reported in data bank. While field data confirmed the molecular results, fully and half-compatible field crosses were not distinguishable. | |
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