ISHS Acta Horticulturae 592: V International Peach Symposium
SCREENING FOR THE MOLECULAR MARKER LINKED TO SAUCER GENE OF PEACH FRUIT SHAPE |
| Authors: | J. Guo, Q. Jiang, K. Zhang, J. Zhao, Y. Yang |
| Keywords: | RAPD, saucer gene, molecular marker, peach |
| Abstract:
By using random primer and bulked segregation analysis (BSA), RAPD genetic markers linked to saucer gene of peach were screened in segregating population of Fuzalode (round) × Wanshudapantao (saucer). High-quality DNA was extracted from young leaves and healthy phloem by using high–salt or cetyltrimethyl ammonium bromide (CTAB) method. Two bulk DNA samples were generated from progeny of Fuzalode × Wanshudapantao. One bulk contained mixed DNA samples of 10 individuals with saucer trait in phenotype, and the other bulk contained 10 non-saucer (round) trait individuals. In addition, a set of RAPD reaction system suitable for peaches was built. The bulks were first used for polymorphism screening with a set of 520 arbitrary 10-mer primers. The two bulks were distinguished by a polymorphic band generate by the RAPD primer OPP09. The size of the polymorphic band was 950bp. The polymorphic fragment was also detected between the parents and their 66 progeny plants. Based on the presence or absence of this polymorphic fragment in the population, it was found that RAPD marker OPP09-950 was linked to the saucer gene in peaches and the linkage distance was 25.4cM. | |
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