ISHS Acta Horticulturae 589: X International Asparagus Symposium
HAPLOID PRODUCTION FROM POLYEMBRYONIC SEEDS OF ASPARAGUS OFFICINALIS L. |
| Authors: | Y. Uno, Y. Ii, M. Kanechi, N. Inagaki |
| Keywords: | dioecy, sex identification, stomata, bulk method, RAPD analysis |
| Abstract:
Asparagus are dioecious and perennial, being grown for 10 years or more. All male cultivars are desired due to higher yields and homogenity, and vigor than cultivars. We attempted to obtain polyembryonic seeds in which haploid seedling might be caught. In addition, searching peculiar genetic markers was attempted on a female and a male bulk and haploid-derived calluses by RAPD (Random Amplified Polymorphic DNA) analysis. The frequency of polyembryonic seeds was 0.34%(34/9925). All polyembryonic seeds had twin seedlings except which one embryonic seed had triplet seedlings. The frequency of polyembryonic seeds was suggested to be different according to the genotypes. The survived 33 seedlings were composed of one haploid (2n=x=10), one triploid (2n=3x=30), and 31 diploids (2n=2x=20). Cladophylls had larger stomata in order of haploid, diploid, and triploid. The size of stomata was less different between haploid and diploid, while larger difference was observed between haploid or diploid, and triploid. RAPD analysis with ten kinds of random primers resulted in occurrence of 3 bands specific to the male bulk and the calluses with the primer No.1, 3 and 5, and one band specific to the female bulk with primer No. 5. | |
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