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ISHS Acta Horticulturae 588: II International Symposium on Cucurbits

IN VITRO CULTIVATION OF CUCUMIS SATIVUS OVULES AFTER FERTILIZATION

Authors:   V. Ondrej, B. Navrátilová, A. Lebeda
Keywords:   Cucumber, ovule culture, double-layer culture medium, zygotic embryogenesis
Abstract:
In vitro ovule culture of Cucumis sativus L. were established on a medium based on the osmotic effect of a double-layer culture medium system. The isolated ovules contained zygotes. The entire process of zygotic embryogenesis was successfully completed and observed by embryological analyses. This process was positively influenced by osmotic effect and coconut water.

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