ISHS Acta Horticulturae 588: II International Symposium on Cucurbits
EFFICIENT PRODUCTION OF DOUBLED HAPLOID MELON PLANTS BY MODIFIED COLCHICINE TREATMENT OF PARTHENOGENETIC HAPLOIDS |
| Authors: | K. Yashiro, K. Hosoya, M. Kuzuya, K. Tomita, H. Ezura |
| Keywords: | Cucumis melo, pure line, X-ray, irradiated pollen, in vitro |
| Abstract:
F1 hybrid cultivars have been widely used for commercial melon (Cucumis melo) production. A pure line is essential for F1 seed production. However, it takes more than seven years to produce such pure lines using conventional plant breeding techniques. Therefore, a method for producing parthenogenetic haploid plants has been developed. In this study, we report a method for efficiently producing doubled haploid (DH) plants by colchicine treatment of haploid plants. Fruits of five F1 cultivars pollinated with soft X-ray (65 KR) irradiated pollen were harvested, and the ovules were cultured in vitro. Fifty-one haploid seedlings were obtained from about 10,000 ovules from twenty fruits in each cultivar. For five cultivars, the rates of haploid production ranged from 0.03% to 0.17%. Twenty seedlings became vitrified or died while growing in vitro. In total, 31 haploid plants were obtained and planted in a greenhouse for doubling. The haploid lines were treated by soaking the tips of many lateral shoots in a 0.1% colchicine solution that contained a small amount of detergent. Of 31 lines, 21 were doubled following colchicine treatment, 68% of the treated plants. | |
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