ISHS Acta Horticulturae 588: II International Symposium on Cucurbits
ELECTROPHORETIC VARIATION FOR SEED PROTEIN OF MUSKMELON GENOTYPES |
| Authors: | S.V. Sawant, T.A. More |
| Keywords: | Cucumis melo, Phoot, C. melo var. momordica, electrophoresis |
| Abstract:
Eight genotypes of muskmelon (Cucumis melo) and Phoot (C. melo var. momordica) genotypes viz. 55-1, 55-2, 77, 113 and 114 identified as resistant sources to downy mildew, PI 124111F and PI 124112 downy mildew resistant lines supplied by Dr. Y. Cohen (Israel) and susceptible cv. M-3 were subjected to study banding pattern of soluble protein, structure and number of bands present in each genotype by using polyacrylamide gel electrophoresis (SDS-PAGE). In total 31 bands were resolved in the gel material of seed protein in different genotypes. Maximum 12 bands in PI 124112 and minimum seven bands in 55-2 and 114 were observed. Band No. 31 was commonly observed in all the genotypes. The differences were observed for banding pattern between downy mildew resistant and susceptible genotypes. Similarly the differences in banding pattern were also observed among the resistant genotypes especially 55-1, 55-2, 113 and 114. All types of bands were observed (dense, medium, light and weak) in all types of genotypes, except 55-2, 113 and 114 which were resistant to downy mildew. Genotypes 55-1 and 55-2 had distinct banding pattern which was different from other resistant genotypes. Genotype 55-2 contained a total of seven bands of which one was dense, four were weak and two were light (Band No. 17, Rm 0.67 and Band No. 20, Rm 0.73). These two light bands were only present in 55-2 and interestingly the medium type band was absent in this genotype. Further this kind of banding pattern noticed in genotype 55-2 was not observed in any other genotypes studied. A maximum similarity index value of 40 per cent was obtained among the pair of genotypes 113 vs 114 indicating close evolutionary relationship among them. Detailed banding pattern of remaining genotypes are discussed in this article. | |
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