ISHS Acta Horticulturae 587: International Symposium on Asian Pears, Commemorating the 100th Anniversary of Nijisseiki Pear
MASS PROPAGATION OF A UNIQUE VARIETY OF PEAR (PYRUS PYRIFOLIA (BURM.) NAK. CV. SEBRI) BY SHOOT TIP CULTURE IN VITRO |
| Author: | M.E. Amiri |
| Keywords: | growth, liquid medium, medium composition, mineral uptake, multiplication, root formation, tissue culture. |
| Abstract:
One of the most popular varieties of pear in Iran is Pyrus pyrifolia (Burm) Nak. cv. Sebri. Fruit features include: excellent eating quality, medium size, light yellow skin color, oval fruit shape, late maturity (mean 220 days), high stone cells content, very juicy, sweet, firm and compact. Potential of Sebri propagation by grafting, or budding is very low and difficult due to incompatibility with the most common rootstocks. Layering or stem cuttings is also difficult to root. Shoot tip of Sebri was cultured on both liquid and gelled modified (zero, fifth, full and twice of mineral concentrations) MS. (Murashige and Skoog, 1962) medium containing BA 5mg/l, IBA 3mg/l, and NAA 5mg/l. Multiplication, growth rate and plant mineral status were influenced by mineral concentration in the medium. Increasing mineral concentration resulted in increased multiplication, growth rate and total mineral uptake by Sebri explants. The highest amount of multiplication (4~5 per month) and the growth rate were obtained in the liquid medium contained high (X2) mineral concentration and 5 mg/l BA. Variation in multiplication and growth of explants can be explained on the basis of water potential and mineral availability to the explants in the liquid medium. | |
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