ISHS Acta Horticulturae 587: International Symposium on Asian Pears, Commemorating the 100th Anniversary of Nijisseiki Pear
IDENTIFICATION OF SELF-INCOMPATIBILITY ALLELES BY S-RNASES SEQUENCING AND PCR-RFLP ANALYSIS IN KOREAN-BRED PEAR (PYRUS PYRIFOLIA) STRAINS |
| Authors: | H.T. Kim, H.J. Kim, I.S. Nou, Y. Hirata, K.K. Kang |
| Keywords: | Pyrus pyrifolia, GSI (gametophytic self-incompatibility), S-RNase, PCR, DNA sequence, specific restriction endonuclease, S-genotype * |
| Abstract:
We interpreted the structure of base sequence on the basis of intron, which is in the hypervariable (HV) region, by analyzing the order of base of S-RNase gene. We found out that the size of the amplified DNA fragments by PCR is different according to the size of the introns from the devised specific primers to S-RNase genes. We analyzed the sequence order of each S-RNase gene, and got the results that there is an intron which exists in the HV region among exons whose homologies are fairly high among those, and the base sequences among S1~S9 RNases are variable. The intron sizes were S1=167 bp, S2=1,153 bp, S3=179 bp, S4=168 bp, S5=179 bp, S6=147 bp, S7=152 bp, S8=234 bp and S9=1,115 bp respectively. In addition the specific restriction endonucleases for each S-allele were selected such as SfcI, XbaI, EcoRI, NdeI, PpuMI, AlwNI, MluI, HinCII, NruI and BstBI. We determined S-genotypes of Korean-bred cultivars and twenty two strains by S-RNase sequencing and PCR-RFLP. From our systematical analysis of Korean-bred pear genotypes by using PCR-RFLP methods, determination of S-genotypes become easier and more convenient, suggesting an applicability to justify incompatible genotypes in other self-incompatible fruit trees. | |
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