ISHS Acta Horticulturae 587: International Symposium on Asian Pears, Commemorating the 100th Anniversary of Nijisseiki Pear
CIS-REGULATORY ELEMENTS FOR PISTIL SPECIFIC EXPRESSION IN S-RNASE PROMOTER REGION OF JAPANESE PEAR (PYRUS PYRIFOLIA NAKAI) |
| Authors: | D.M.R.K.K. Dissanayake, S. Norioka, N. Norioka, T. Takasaki, T. Nakanishi |
| Keywords: | Pyrus pyrifolia, self-incompatibility, deletion promoter, GUS, transgenic plant |
| Abstract:
The pistil-specific and temporal expression of Japanese pear S-RNase gene is regulated by S-RNase promoter region. To identify the cis-regulatory elements on the S-RNase promoter of Japanese pear, we analysed GUS activities in transgenic tobacco plants carrying 9 sequential deletions of S3-RNase promoter fused to the  -glucuronidase (GUS) gene.
GUS activity was detected quantitatively in the pistils from the transgenic plants carrying the promoter deleted from -747 to -310. GUS activity was localized in the stigmatic secretory zone, and the transmitting tissues in the pistils but not in other tissues; pollen, anthers, petals, sepals, leaves and roots.
GUS activities in the pistils increased steadily from 4 days before flowering.
No transgenic pistils with deletions carrying -255 or shorter promoters displayed GUS activity, indicating that the critical cis-regulatory elements for pistil specific expression were present between -310 and -255 of S3-RNase promoter region.
Furthermore, deletions from -552 to -356 led to the drastic decrease of GUS activity in the transgenic pistil, which assumes that enhancer-like element for the high level of expression in pistils are present within this region.
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