ISHS Acta Horticulturae 587: International Symposium on Asian Pears, Commemorating the 100th Anniversary of Nijisseiki Pear
PHYLOGENETIC ANALYSIS OF PYRUS SPECIES: THE CHLOROPLAST DNA STRUCTURE OF PYRUS USSURIENSIS VAR. HONDOENSIS |
| Authors: | H. Katayama, C. Uematsu |
| Keywords: | Pyrus ussuriensis, Rosaceae, chloroplast DNA, structural alteration, deletion, phylogeny |
| Abstract:
To investigate the phylogenetic relationships in Pyrus and other species in the Rosaceae, structure of chloroplast DNA (cpDNA) in pear (Pyrus ussuriensis var. hondoensis (Nakai et Kikuchi) Rehder) was compared with that of tobacco in Solanaceae and those of Gramineous plants. Twenty-nine genes were mapped onto pear cpDNA by Southern hybridization. The gene order in pear cpDNA was the same as those for tobacco and Arabidopsis thaliana cpDNA, but differed from those for Gramineous plants which have large deletions (the regions including some genes) and inversions compared with pear and tobacco cpDNA. Also the structure of pear cpDNA was different from that of Lotus japonicus and Oenothera elata with a large inversion in the region between rps16 and rbcL. Three deletions were detected in the non-coding region between rbcL and accD in pear cpDNA when compared with tobacco cpDNA. Distribution of these deletions were surveyed by PCR in 20 Rosaceous plants including 11 Pyrus species from subfamily Maloideae, and 9 other species from subfamilies Prunoideae, Rosoideae, and Spiraeoideae. These deletions were found in all of the Rosaceous plants except 5 Prunus species, suggesting that the deletions occurred before the differentiation of Rosaceae, and additional alterations might occur in Prunus species. | |
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