ISHS Acta Horticulturae 587: International Symposium on Asian Pears, Commemorating the 100th Anniversary of Nijisseiki Pear
UTILIZATION OF PCR-FINGERPRINTING FOR CLASSIFICATION OF KOREAN PEAR CULTIVARS |
| Authors: | J.H. Oh, C.S. Choi, K.K. Kang, I.S. Nou, M.Y. Eun, H.W. Kang |
| Keywords: | PCR-fingerprinting, Genetic distance, Classification, Polymorphism |
| Abstract:
PCR-fingerprinting analysis was applied to classify and identify twenty-five pear cultivars from throughout South Korea. Among 12 primers (21mer) used for this experiment, only 10 primers revealed clear and reproducible band patterns, and produced 36 polymorphic bands among 97 bands. Based on the presence of polymorphic bands, the value of the co-phenetic correlation coefficient, a measure of the goodness of fit between the dendrogram and the original similarity matrix, was 0.20. The cultivars were classified by UPGAM clustering method into four main groups. The first group included five cultivars (‘Ohsaeshipsegi’, ‘Singo’, ‘Shinhong’, ‘Shinil’, ‘Jangshimnang’). ‘Hangsu’, Josangjuk’, Wonhwang’, ‘Kamchonbea’, ‘Mihwang’ and Youngsanbea belonged to the second cluster. The third group had ten cultivars (‘Chisung’, ‘Eshipsegi’, ‘Hwangkmbea’, ‘Sunhwang’, Hwasan’, ‘Kamro’, ‘Mansu’, ‘Chungsilri’, ‘Mansamkil’ and ‘Sinsu’). ‘Pungsu’, ‘Kumchunchu’ and ‘Kunjongchonsang’ belonged to the fourth cluster. From these results, we concluded that there was no correspondence between fruit morphological characteristic and their genetic fingerprint. By contrast the local varieties, allied varieties by the similarity coefficient, were closely related to the distributed areas. | |
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