ISHS Acta Horticulturae 587: International Symposium on Asian Pears, Commemorating the 100th Anniversary of Nijisseiki Pear
THE JAPANESE PEAR GENOME PROGRAM II. GENETIC LINKAGE MAP IN PEAR |
| Authors: | T. Yamamoto, H. Iketani, Y. Sawamura, T. Kimura, T. Imai, T. Saito, M. Shoda, K. Kotobuki, T. Hayashi, N. Matsuta |
| Keywords: | Pyrus pyrifolia, Pyrus communis, simple sequence repeats, mapping, disease resistance |
| Abstract:
Pear genome research has been done using genetic linkage maps of Japanese and European pears, in the program of the National Institute of Fruit Tree Science, Japan. Two disease-related genes to black spot and pear scab diseases were identified in genetic linkage maps of ‘Kinchaku’ and ‘Kousui’. Several RAPD markers linked to the disease resistance (or susceptibility) loci could be obtained, which will be used in marker assisted selection. The genetic linkage maps of the European pear ‘Bartlett’ and the Japanese pear ‘Housui’ were also constructed with DNA markers including AFLPs and SSRs originated from pear and apple. The former map consisted of 19 linkage groups that covered more than 1300 cM. Segregation of almost all SSR markers fitted the expected ratio of monogenic inheritance and SSR loci could be used as anchor markers to join different maps. Interestingly, SSRs developed from apple produced discrete amplified bands in pear and were mapped in pear linkage maps. Sequencing analysis showed that the nucleotide sequences of SSR alleles in pear and apple were highly homologous, and that SSR loci were presumably generated from the same origin. | |
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