ISHS Acta Horticulturae 587: International Symposium on Asian Pears, Commemorating the 100th Anniversary of Nijisseiki Pear
THE JAPANESE PEAR GENOME PROGRAM I. DEVELOPMENT OF SSR MARKERS AND IDENTIFICATION OF PEARS |
| Authors: | T. Kimura, Y. Ban, T. Yamamoto, Y.Z. Shi, K. Kotobuki, N. Matsuta, T. Hayashi |
| Keywords: | genetic diversity, microsatellites, mutant, Pyrus spp., simple sequence repeat |
| Abstract:
Thirty-five SSRs were developed in pear by using 3 different approaches, RAHM (random amplified hybridization microsatellites), 5’ anchored PCR methods and an enriched genomic library. Genetic diversity of 57 pear varieties including 22 Japanese, 20 Chinese and 12 European pears, was identified by 11 SSR loci. These SSR markers could be utilized on 5 tested Pyrus species. All SSR primers could produce 1 or 2 discrete amplified fragments in all tested accessions. Fifty-seven pear varieties from 4 species were successfully differentiated together using a total of 142 alleles. The number of alleles per locus ranged from 4 for KA4b to 19 for NH014a, with an average value of 12.9. Variation of genotype was observed within species as well as among species. In 3 Asian species (P. pyrifolia, P. bretschneideri and P. ussuriensis), about 24~41% of the alleles were shared for each pair of species. Mutant varieties and their original cultivars were analyzed by 11 SSRs. Identical patterns were obtained between mutants and their original cultivars. In addition, 32 individuals of ‘Choujuurou’ from different sources also showed the same genotypes with 11 SSR loci. | |
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