ISHS Acta Horticulturae 586: IV International Symposium on Olive Growing
APPLICATION OF MICROSATELLITE MARKERS IN OLIVE BREEDING |
| Authors: | P. Rallo, G. Dorado, A. Martin |
| Keywords: | Olea europaea, SSR, VNTR, PCR, codominancy, biodiversity, paternity |
| Abstract:
Five microsatellites recently developed for olive tree (Olea europaea L.) have been tested as useful markers for different breeding purposes: species and cultivar identification, testing intra and interspecific variability, linking ligation maps and performing paternity analysis. Microsatellite amplification has been tested in 33 genotypes of 15 Olea species other than Olea europaea. At least 12 of them succesfully amplified for the four more polymorphic loci. Polymorphism was found at inter and intra specific level, confirming its usefulness as a tool for biodiversity studies. Cultivar identification could be achieved through these microsatellites in a set of of 46 cultivars. Ninety-five per cent of them were fully identified with just as few as three microsatellites. Mendelian segregation was checked in a ‘Leccino’x’Dolce Agogia’ population in three loci for which parents were polymorphic. Paternity analysis have been performed in recently developed populations: ‘Arbequina’x’Picual’, ‘Arbequina’ x ‘Zaity’, ‘Picual’x’Lechín de Sevilla’ and ‘Picual’ x ’Zaity’. A fluorescent system based on ABI 310 sequencer (PE Biosystems) has been optimized for resolving amplification products and precise allele size determination. When performing paternity analysis, amplification products have been resolved in Metaphor agarose gels, where alleles can be easily scored. | |
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