ISHS Acta Horticulturae 586: IV International Symposium on Olive Growing
CHARACTERISATION OF OLIVE (OLEA EUROPAEA L.) CULTIVARS BY RAPD MARKERS |
| Authors: | D. Bandelj, J. Jakże, B. Javornik |
| Keywords: | polymorphism, RAPD, Olea europaea, Slovenia, Slovene Istra |
| Abstract:
The aim of our research was to screen intra- and inter- cultivar genetic polymorphism of olive cultivars by molecular markers. Random Amplified Polymorphic DNA of five local cultivars ('Istrska belica', 'Štorta', 'Buga', 'Crnica', ‘Unknown 2’) and ten foreign cultivars ('Leccino', 'Nocellara del Belice', 'Frantoio', 'Cipressino', 'Santa Caterina', 'Pendolino', 'Arbequina', 'Maurino', 'Picholine', 'Unknown 1') was analysed, using thirteen different arbitrary primers: OPA-1, OPA-4, OPA-8, OPX-2, OPX-3, OPX-4, OPX-7, OPX-8, OPX-11, OPX-12, OPX-13, OPX-14, OPX-19. RAPD data was used for clustering analysis to assess the genetic reletionships among cultivars analysed. The results showed high genetic polymorphism among cultivars and high similarity between cultivars in the collection orchard and orchards of Slovene Istra. | |
Download Adobe Acrobat Reader (free software to read PDF files) | |
