ISHS Acta Horticulturae 586: IV International Symposium on Olive Growing
MEDIUM- AND LONG- TERM IN VITRO CONSERVATION OF OLIVE GERMPLASM (OLEA EUROPAEA L.) |
| Authors: | M. Lambardi, C. Benelli, A. De Carlo, A. Fabbri, S. Grassi, P.T. Lynch |
| Keywords: | germplasm preservation, slow growth storage, cryopreservation, vitrification |
| Abstract:
The potential for the conservation of olive germplasm by both slow growth storage (4°C), and preservation in liquid nitrogen was examined. For medium-term storage, olive shoot cultures (cvs Leccino and Frantoio) were effectively preserved in the dark at 4°C for up to 8 months. With cryopreservation based long-term storage, best results were obtained with portions of embryogenic tissue (cv Canino), which exhibited post-thaw regrowth and production of somatic embryos after the application of controlled rate freezing or vitrification procedures. A maximum of 15% post-thaw survival was obtained by using the vitrification/one-step freezing procedure with shoot tips from in vitro-grown plantlets of the cv Frantoio. | |
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