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ISHS Acta Horticulturae 579: II Balkan Symposium on Vegetables and Potatoes

PRESOWING APPLICATION OF GIBBERELLIC ACID ON SEEDS USED FOR THE MUNG BEAN BIOASSAY AFFECTS HORMONE CONTENT OF CUTTINGS

Authors:   M. Koukourikou-Petridou, I.C. Porlingis
Keywords:   Hypocotyl, paclobutrazol, root formation, sucrose, Vigna radiata (mung bean), gibberellic acid, indole-3-acetic acid, cytokinins
Abstract:
Cuttings of mung bean plants [Vigna radiata (L.) R. Wilcz] derived from seeds that have accepted the presowing effect of GA3 contained higher levels of free indole-3-acetic acid (IAA) and lower ones of cytokinins than the control, the first two days after excision. Treatment of seeds with GA3 and cutting treatment with synthetic IAA, paclobutrazol (PBZ) or sucrose just after cutting excision, for 24 h, resulted in an increase in the root number per cutting. A significant interaction in the root formation was observed between presowing treatment with GA3 and cutting treatment with PBZ or sucrose. The interaction between cutting treatment with IAA and PBZ or IAA and sucrose was significant also. The interaction of GA3 (presowing application) with PBZ or sucrose (cutting treatment) was stronger than that of IAA with PBZ or sucrose (all three substances were applied to cutting bases). The results showed that the effect of GAs on root formation was only partially due to the increase of endogenous IAA content in the base of the cuttings.

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