ISHS Acta Horticulturae 579: II Balkan Symposium on Vegetables and Potatoes
COMBINATION OF MICROPROPAGATION WITH MICROGRAFTING IN CUCUMBER |
| Authors: | G. Sapountzakis, A.S. Tsaftaris |
| Keywords: | Cucumis sativus, in vitro regeneration, shoot organogenesis |
| Abstract:
In an effort to combine micropropagation with micrografting in cucumber, seeds of the cucumber hybrid Stella were surface sterilized and placed on 0.8 % w/v agar in darkness for one week to germinate. One-week-old seedlings were used as donors of cotyledon explants. Cotyledons were divided in two segments: the proximal and the distal ones in comparison to the embryonic axis. The proximal segments were cut in two parts along the central vein. These parts were cultivated on MS medium supplemented with 0.1 mg/L BA. Shoots, which were regenerated from these explants, were transferred to MS medium where they elongated and rooted. Every three weeks plantlets were cut in one-bud explants and transferred to new media where they were developed again in plantlets with micropropagation rate 1:6. Seeds of the cucumber rootstock ΤΖ 148 were surface sterilized and placed on 0.8 % w/v agar in darkness for one week to germinate. One-week-old rootstock seedlings were transferred to MS medium. Two-weeks-old rootstock seedlings were micrografted with a micropropagated shoot of ‘Stella’ (derived from cotyledon explant regeneration). Subsequently the micrografted plants were transferred to fresh MS media. Two weeks later plants were transferred progressively to in vivo conditions. | |
Download Adobe Acrobat Reader (free software to read PDF files) | |
