SANITARY STATUS OF PLUM VARIETIES AT THE KYUSTENDIL REGION OF BULGARIA

Authors:	I. Kamenova, A. Borisova
Keywords:	PNRSV, PDV, ACLSV. PPV, PPV strains, monoclonal antibodies
Abstract: The aim was to assess the sanitary status of plum varieties in one of the main stone fruit growing area of Bulgaria. Different plum varieties from two experimental and one commercial orchards were investigated for the most important stone fruit viruses as plum pox (PPV), prunus necrotic ringspot (PNRSV), prune dwarf (PDV) and apple chlorotic leaf spot (ACLSV). The studies were based on: Field surveys for the observation of symptoms; DAS-ELISA test for virus identification; DASI-ELISA test for PPV strain characterization. No ACLSV was recorded, while the rate of PPV, PNRSV and PDV infections were 63.2%, 11.04 and 1.8%, respectively. In addition strain characterisation and differentiation of PPV isolates by the use of monoclonal antibodies revealed that the most distributed strain was PPV-M (74.8%), followed by mixed M+D and PPV-D infections (13.6% and 11.6%), respectively.
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