ISHS Acta Horticulturae 577: VII International Symposium on Plum and Prune Genetics, Breeding and Pomology
POSSIBILITIES FOR APPLICATION OF PHOTOAUTOTROPHY IN MICROPROPAGATION OF DZHANKA 4 (PRUNUS CERASIFERA EHRT.) ROOTSTOCK |
| Authors: | L. Nacheva, K. Ivanova, P. Manolov, Z. Zlatev |
| Keywords: | net photosynthetic rate, Prunus cerasifera Ehrt., quantum yield, Rubisco activity, rooting |
| Abstract:
In vitro cultured plantlets have heterotrophic development, relying on the use of exogenous sugars as a carbon source for growth. During the acclimatization stage, plantlets are constrained to grow photoautotrophically, i.e. to synthesize carbohydrates through their own photosynthesis. Many researchers suggested that the photosynthetic competence of plants, when transferred to soil, might be an important factor in determining transplant survival. The aim of the present investigation was to study photosynthetic ability and possibilities for application of photoautotrophy during micropropagation of Dzhanka 4 (Prunus cerasifera Ehrt) rootstock. Observations were made on net photosynthesis of plantlets at three stages of micropropagation (multiplication, elongation and rooting), cultured under conventional conditions and conditions favouring photosynthesis (light, gas-permeable closure and decreased sucrose concentration). In addition, the same photosynthetic characteristics were analyzed in photoautotrophically-rooted (in peat) plantlets. The improved environmental conditions led to an increase of quantum yield and Rubisco activity. In result, the net photosynthetic rate increased by 63% (in multiplication) to 91% (in rooting). The results from this research and the recent experiments showed that improved environmental conditions enhanced photosynthesis and enabled the application of photoautotrophy in micropropagation of Prunus cerasifera Ehrt. | |
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