ISHS Acta Horticulturae 576: International Conference on Medicinal and Aromatic Plants. Possibilities and Limitations of Medicinal and Aromatic Plant Production in the 21st Century
INHIBITORY EFFECT OF ACORUS CALAMUS L. EXTRACT ON SOME PLANT PATHOGENIC MOLDS |
| Authors: | P. Mungkornasawakul, D. Supyen, C. Jatisatienr, A. Jatisatienr |
| Keywords: | sweet flag, fungicide, medicinal plant, essential oil, β-asarone, antimicrobial effect |
| DOI: | 10.17660/ActaHortic.2002.576.51 |
| Abstract:
Dry and powdered rhizomes of Acorus calamus L. were extracted with ultrasonic bath using dichloromethane as solvent. Various concentrations (0.01-0.15 %) of the extract were determined for antifungal activity on PDA agar against Alternaria spp. isolated from leaf spot and Fusarium spp. isolated from wilt diseases of cruciferous vegetable, as well as Botrytis spp. isolated from gray mold rot of roses and Septoria spp. isolated from leaf spot of chrysanthemum. The results indicated that all of the molds examined were sensitive to Acorus calamus extract. The growth of all tested fungi was completely inhibited at the concentration of 0.10 % upward. Separation by preparative-TLC and guidance by TLC-bioassay using Cladosporium cladosporioides as a diagnostic fungus revealed an active compound that was identified as  -asarone (cis-1,2,4-trimethoxy-5-(1-propenyl)-benzene) by GC-MS.
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