ISHS Acta Horticulturae 575: International Symposium on Tropical and Subtropical Fruits
PROGRESS IN THE DEVELOPMENT OF TRANSGENIC PAPAYA: TRANSFORMATION OF SOLO PAPAYA USING ACC SYNTHASE ANTISENSE CONSTRUCT |
| Authors: | P.M. Magdalita, A.C. Laurena, B.M. Yabut-Perez, J.R. Botella, E.M. Tecson-Mendoza, V.N. Villegas |
| Keywords: | Transformation, microprojectile bombardment, somatic embryos, ACC synthase |
| Abstract:
Papaya (cv. Solo) somatic embryos were produced, squashed and used for transient stable transformation. Conditions for transforming somatic embryos were optimized using transient expression of GUS. A total of 11,900 immature zygotic embryos were isolated and 7,730 (65%) produced somatic embryos. All the somatic embryos were squashed, placed in an osmotic medium and bombarded with the antisense ACC synthase construct placed in a co-transformation vector. A total of 160 bombardments were made. The bombarded tissues were subjected to a selection medium containing half-strength MS with 150 mg L-1, then with 300 mg L-1 kanamycin. Embryos surviving kanamycin-selection were regenerated on a full strength De Fossard medium supplemented with 0.25 μM each of BAP and NAA and 10.0 μM GA3. | |
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