ISHS Acta Horticulturae 572: XX International Eucarpia Symposium, Section Ornamentals, Strategies for New Ornamentals - Part II
TRANSFORMATION OF GERBERA PLANTS WITH TOMATO SPOTTED WILT VIRUS (TSWV) NUCLEOPROTEIN GENE |
| Authors: | M. Korbin, M. Podwyszynska, B. Komorowska, D. Wawrzynczak |
| Keywords: | transformation, gerbera, TSWV nucleoprotein gene |
| Abstract:
Tomato spotted wilt virus (TSWV) is identified as a causal agent of an important disease in many vegetable and ornamental crops. In the Research Institute of Pomology and Floriculture, studies on the introduction of resistance-related, TSWV nucleoprotein gene to the gerbera genome (‘Alaska’, ‘Paul', ‘Prince’, ‘Zuzanna’) were undertaken. Gerbera explants (shoots, bases of shoot clumps and leaves with 2-3 mm lamina length) were transformed by Agrobacterium tumefaciens (strain LBA 4404). Construction of the expression vector containing the 35S CaMV promoter, the selectable nptII gene and the N-gene of the virus naturally infecting gerbera was based on pBin19 plasmid. After 10 months, seventy-four (5.4%) transgenic gerbera plants were selected on medium with the maximal concentration of kanamycin (7 mg l -1). The presence and integration of the N-transgene into the plant genome was confirmed by specific-PCR and Southern-blotting. The level of nucleoprotein estimated by ELISA was low in transformed tissues, but mechanically inoculated, transgenic plants demonstrated resistance to TSWV. | |
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