ISHS Acta Horticulturae 570: VIII International Symposium on Flowerbulbs
ANATOMICAL CHANGES IN GLORIOSA ROTHSCHILDIANA O'BRIEN STEMS INDUCED BY JA-ME AND ABA |
| Authors: | E. Weryszko-Chmielewska, D. Kozak |
| Keywords: | ABA, anatomy, Gloriosa rothschildiana, JA-Me, stem |
| Abstract:
Tuberization of Gloriosa rothschildiana ‘Red Dark’ is inhibited in the presence of different concentrations of ABA and higher levels of JA-Me. Anatomical changes in stems accompanying the inhibition of tuber development in in vitro culture were studied. Murashige and Skoog (MS) medium containing 6% of sucrose and 5 mg l-1, 25 mg l-1 JA-Me or ABA at concentration of 0.01 mg l-1 was used. Stem tissues after a 4-week incubation period on medium were examined. Both growth regulators caused the decrease of parenchyma cells size. JA-Me concentrations invoked the increase of number and diameter of xylem vessels, but ABA a strong reduction of vessel number. At 25 mg l-1 JA-Me, local necroses in parenchyma were observed. Histological changes found corresponded with weaker formation of tubers at ABA presence and higher JA-Me concentration. | |
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