ISHS Acta Horticulturae 567: IV International Strawberry Symposium
GENETIC STABILITY OF PURE LINES OF FRAGARIA VESCA L. IN MICROPROPAGATION AND LONG-TERM STORAGE IN VITRO |
| Author: | T. Morozova |
| Keywords: | genetic markers, regeneration capacity, apical meristems, leaf explants, field tests, seed progeny |
| Abstract:
The genetic stability of 12 pure lines of Fragaria vesca L. has been studied. The plants were either grown directly from seeds (control) or micropropagated through meristem culture, or regenerated from leaf explants. Half of the seed-derived plants were subjected to long-term cold storage in vitro. The effect of experimental procedures on the stability of morphological characteristics was evaluated on field-grown plants and on their self-pollinated progenies. Specimens with abnormal leaf morphology were absent among control and cold-stored plants, but accounted for 9 % of meristem-micropropagated plants and 21 % of plants regenerated from leaf explants. The lines varied significantly in callus-forming and regeneration capacities and in the frequency of somaclonal variation. | |
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