ISHS Acta Horticulturae 567: IV International Strawberry Symposium
ISOZYME AND RAPD CHARACTERIZATION OF WILD AND CULTIVATED NATIVE FRAGARIA IN SOUTHERN CHILE |
| Authors: | M. Gambardella, A. Cadavid-Labrada, V. Díaz |
| Keywords: | Chile, strawberry, Fragaria chiloensis, cultivar identification, DNA, molecular markers, RAPD, isozyme |
| Abstract:
One hundred two accessions of wild and cultivated Fragaria chiloensis, collected in southern Chile, and were studied. They were characterized using isozymes and RAPD markers. The isozymes used were PGI, LAP, EST and PGM and were separated by horizontal starch gel electrophoresis (Tanksley and Orton, 1983). Polymorphism was observed in zymograms obtained for all enzyme systems. Using RAPD markers, from 80 Operon primers, 17 were selected because they provided a greater number of polymorphic bands. The information was used to evaluate the genetic variability among wild clones. The results indicated that there exists a high degree of genetic variability among the different accessions and that four genetically related groups can be identified. This was also corroborated by the morphological characterization. | |
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