ISHS Acta Horticulturae 567: IV International Strawberry Symposium
PLANT REGENERATION FROM LEAF EXPLANTS IN STRAWBERRY (FRAGARIA x ANANASSA DUCH.) |
| Authors: | J.I. Zebrowska, J. Hortynski |
| Keywords: | tissue culture, shoot regeneration, leaf blades, petioles, 6-benzylaminopurine |
| Abstract:
Plant regeneration from leaf explants i.e. petioles and leaf blades at various concentrations of 6-benzylaminopurine (BAP) : 0; 1.6; 3.2; 6.4 mg/litre in Murashige –Skoog (MS) medium in clone B-302 and cultivar Kama was examined. Leaf explants regenerated only at concentrations of 3.2 mg and 6.4 mg BAP in the medium. Shoot formation was better in clone B-302 than in cultivar Kama . Regeneration from petioles of clone B-302 was the best and reached 30% and 17% from leaf blades with an average of 6 and 8 shoots per explant (respectively) at 3.2 mg of BAP. At the same concentration of BAP in MS medium no shoot formation was observed in cultivar Kama which regenerated only at concentration of 6.4 mg BAP. Generally, in both genotypes, regeneration from petioles was better than from leaf blades although number of plantlets per leaf blade was higher when compared with that obtained from petioles. | |
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