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ISHS Acta Horticulturae 567: IV International Strawberry Symposium

THE PHOTOSYNTHETIC STATUS OF IN VITRO STRAWBERRY SHOOTS

Authors:   E. Zenkteler, B. Borkowska
Keywords:   Fragaria x ananassa, morphology, photosynthesis, shoot cultures, sugars, chloroplasts ultrastructure
Abstract:
The last phase of multiplication of strawberry (before rooting ex vitro) was carried out on Lee and De Fossard medium (rich in macroelements) and supplemented with either glucose or sucrose (30 g L-1). Chlorophyll content and value of Fv/Fm were higher in leaves from sucrose containing medium than in leaves from glucose-medium. Two other chlorophyll fluorescence parameters (Fs and Rfd) revealed a better balance between light and dark phases of photosynthesis in glucose-cultivated shoots. At the end of the final culture period, medium was free of sugars when glucose was supplied and still abundant in carbon for the sucrose-medium. There were observed irregularities in ultrastructure of chloroplasts from leaves of both treatments, manifested by swelling and dilation of stroma thylakoids and unstacking or decline of the grana thylakoids. These symptoms were more obvious in leaves from sucrose containing medium.

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