ISHS Acta Horticulturae 567: IV International Strawberry Symposium
PHYLOGENETIC STUDIES ON FRAGARIA SPP. BY USING RANDOM AMPLIFIED HYBRIDIZED FRAGMENT POLYMORPHISM (RAHFPs) |
| Authors: | G. Martelli, M.R. Sabina, F. Sunseri, I. Greco |
| Keywords: | strawberry, genetic similarity, phylogenetic analysis |
| Abstract:
The cultivated strawberry (Fragaria x ananassa Duch.) is an octoploid species, which originated by natural hybridization between wild octoploid Fragaria species. With the aim to understand better the genomic structure of the cultivated strawberry, the genetic relationship among the octoploid Fragaria species was investigated. Three wild species (F. virginiana L., F. chiloensis L. and F. virginiana glauca L.) and 18 strawberry cultivars, coming from different breeding programs, were utilized in this study in three different steps: A) the optimization of RAPDs protocol and the setup of a new class of molecular marker (RAHFPs); B) the evaluation of genetic relationships in wild octoploid species; C) the evaluation of genetic contribution of the investigated genomes in the cultivated strawberry. Starting from 92 random primers 10bp, several DNA fragments were obtained and 84 out of 213 were utilized like probes. The hybridizations were performed either on amplification products or on digested genomic DNA fragments. The polymorphisms among the genotypes were transformed into numeric values and used to generate a genetic similarity index (DICE). The results obtained indicated that the class of molecular marker named RAHFPs are more informative then RAPDs. Moreover, the clusters generated from RAPDs and RAHFPs data were differentially informative in relation to the phylogenesis of the analyzed genotypes. | |
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