ISHS Acta Horticulturae 560: IV International Symposium on In Vitro Culture and Horticultural Breeding
INDUCTION AND CHARACTERIZATION OF CALLUS IN HALOXYLON APHYLLUM (MINKW.) ILJIN |
| Authors: | A.E. Ramirez, E.H. Birnbaum |
| Keywords: | black saxual, somatic embryogenesis, auxin, cytokinin, suspensor |
| Abstract:
Callus from mature zygotic embryos of Haloxylon aphyllum was induced on Burnouf-Radosevich-Paupardin (BRP) medium supplemented with different concentrations (0.1, 1.0, 5.0 mg/l) of 2,4-dichlorophenoxyacetic acid (2,4-D), naphthalene acetic acid (NAA), indole-3-acetic acid (IAA) or indole-3-butyric acid (IBA) alone or combined with 0.1 mg/l 6-benzyladenine (BA) or kinetin (Kin). The highest frequency of callus induction and greatest amount of callus proliferation occurred on BRP medium supplemented with 1.0 mg/l 2,4-D combined with 0.1 mg/l BA. Following transfer to BRP medium without growth regulators, cell clusters proliferated on the surface of the callus and groups of elongated suspensor cells developed from the clusters. The morphology of the cell clusters and the suspensor-like cells that developed from them support the hypothesis that the tissue is embryogenic callus. | |
Download Adobe Acrobat Reader (free software to read PDF files) | |
