ISHS Acta Horticulturae 560: IV International Symposium on In Vitro Culture and Horticultural Breeding
INFLUENCE OF GELLING AGENTS ON ILEX PARAGUARIENSIS TISSUE CULTURE |
| Authors: | P.A. Sansberro, H.Y. Rey, C.V. Luna, L.A. Mroginski |
| Keywords: | Ilex paraguariensis, tissue culture, gelling agents |
| Abstract:
The effect of seven gelling agents (Agar A-1296; Agar A-9799; Agar A-7921; Agarose A-0169; Agargel A-3301, Transfergel T-5660, and Phytagel P-8169. All of them from Sigma Chemical Co.) on different in vitro tissue culture systems of Ilex paraguariensis was studied. Interactions were found between type of agar and plant regeneration. The type of agar, being Phytagel significantly better than Agar (A-1296) affected somatic embryogenesis by in vitro culture of immature embryos. However, Agargel promoted induction of callus from leaves, while Phytagel was the best gelling agent for callus growth. Although, the type of gelling agent did not affect the in vitro establishment of nodal segments, the multiplication phase as well as the rooting of regenerated shoots was greatly increased by Phytagel. Phytagel was also found to be the more adequate gelling agent for in vitro maturation and germination of immature zygotic embryos. | |
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