ISHS Acta Horticulturae 560: IV International Symposium on In Vitro Culture and Horticultural Breeding
THE INFLUENCE OF EXOGENOUS CYTOKININS ON THE PROLIFERATION OF EMBRYOGENIC TISSUE AND SOMATIC EMBRYO MATURATION OF NORWAY SPRUCE |
| Authors: | M.J. Latkowska, H. Chmiel, K. Molska |
| Keywords: | Picea abies, plant growth regulators, somatic embryogenesis, 2iP, thidiazuron |
| Abstract:
The influence of exogenous cytokinins on the proliferation of embryogenic tissue (ET) and subsequent somatic embryo maturation of three genotypes of Norway spruce, with different capacities for mature somatic embryo production, was studied. ET was cultured on the BMI-S1 medium supplemented with 5 μM 2,4-dichlorophenoxyacetic acid (2,4-D) and either thidiazuron (TDZ) or 2-isopentenyladenine (2iP) (0.5; 2.5; 5 or 10 μM). The BMG-1 medium supplemented with 10 or 40 μM abscisic acid (ABA) was used for somatic embryo maturation. Effect of the cytokinin on ET proliferation and somatic embryo maturation was genotype dependent. The fastest growing BLG-10 was very efficient in cotyledonary embryo production in all treatments. However, 2iP strongly inhibited tissue growth and production of mature somatic embryos in this genotype. 2iP stimulated embryo maturation in 186-5, in the presence of 40 μM ABA. | |
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