ISHS Acta Horticulturae 560: IV International Symposium on In Vitro Culture and Horticultural Breeding
IN VITRO STUDIES ON CALLUS INDUCTION IN BOTH VEGETATIVE AND GENERATIVE PARTS IN ALSTROEMERIA FOR FURTHER APPLICATION TO TRANSFORMATION |
| Authors: | J.B. Kim, M. de Jeu, K.J.J.M. Raemakers, E. Jacobsen, R.G.F. Visser |
| Keywords: | Compact callus, friable callus, monocot, regeneration, transformation |
| Abstract:
We compared both vegetative and generative parts of Alstroemeria in friable embryogenic callus induction, and subsequent production of somatic embryos and regeneration of plants because there exist only a few protocols with low efficiency for application to transformation. Nodal parts of the plant showed the best performance in callus induction, production of somatic embryos and regeneration as compared to internodes and all seven generative parts tested. Thus, it can be concluded that the nodal explants can be used as a source for transformation in combination with the MS medium containing 1 mg/l 2,4-D, 0.25 mg/l BAP, 3% sucrose (w/v) and 0.75% (w/v) microagar for the production of a high level of compact callus and somatic embryos. In the future, more research will be needed on the acceleration of the plant production process from somatic embryos, to use this system in an optimised way for transformation experiments. | |
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