ISHS Acta Horticulturae 560: IV International Symposium on In Vitro Culture and Horticultural Breeding
EFFECTS OF ABA, FLURIDONE AND LIGHT QUALITY ON GROWTH AND DORMANCY OF TISSUE-CULTURED HERBACEOUS PEONY SHOOTS |
| Author: | E. Gabryszewska |
| Keywords: | peony, ABA, fluridone, light quality, in vitro, dormancy |
| Abstract:
The influence of ABA 1 mg l-1, fluridone 1 mg l-1 and light quality (white, red, blue) on the growth and dormancy of non-dormant and dormant peony cv. Jadwiga shoots grown on the media without or with cytokinins (BAP+2iP+kinetin+thidiazuron) were investigated. In the shoots that became dormant, the rate of growth and the rate of multiplication were reduced. Also, senescence and death of the leaves took place. The application of ABA or fluridone to the cytokinin-free medium had no effect on the number of axillary shoots but influenced the leaf formation on non-dormant and dormant shoots grown under all light qualities. The addition of ABA with cytokinins inhibited the leaf production in non-dormant as well as dormant shoots. Fluridone, an inhibitor of ABA-synthesis, applied during culture, promoted leaf formation and axillary shoot production. The activity of fluridone in leaf and shoot formation was modified by light quality. The highest number of leaves and shoots were found on the explants grown under red light both on non-dormant and dormant shoots. The inhibition of leaf formation by ABA and the opposite effect of fluridone in this processes suggest that development of dormancy in tissue-cultured peony shoots can be caused by ABA. | |
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