ISHS Acta Horticulturae 560: IV International Symposium on In Vitro Culture and Horticultural Breeding
IN VITRO CULTURE OF HUNGARIAN APRICOT (Prunus armeniaca L.) VARIETIES |
| Authors: | I. Balla, J. Vértesy |
| Keywords: | micropropagation, apricot varieties, virus elimination, BAP-ribosid, elongation |
| Abstract:
Apricot is one of the most important fruit crops in Hungary, with a production of 40000 t/year. Hungary, like most of the Europian countries is strongly infected with Plum pox virus. The virus elimination till now happened by thermotherapy in vivo. This method is very effective, but time-consuming. To shorten the time of virus elimination the in vitro thermotherapy is planned, as first step to it the development of sterile propagation method of the apricot varieties. Actively growing shoot-tips of the required varieties were collected for initiation of the culture. Following a desinfection procedure 70 % of the shoots begin to grow under sterile condition. The growing shoots have to be transferred into fresh propagation medium every 3 weeks and kept in a growth room of 22 °C, under 16/8 h photoperiod with a light intensity of 1500 Lux. The typical propagation rate of the different varieties is reached 3-4 months following the start of induction, differing from 3 to 8. An elongation medium with a relatively high IAA concentration is neccesary to use before rooting. The elongated, about 20 mm long shoots should be rooted on a half strength modified medium. The rooted shoots can be acclimatized under high humidity to the greenhouse condition. The development of the micropropagation method of different apricot varieties is a good opportunity to begin the in vitro thermotherapy trials to get virusfree plants as well as chould be a basis for regeneration experiments in genetic transformation against Plum pox virus. | |
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