ISHS Acta Horticulturae 560: IV International Symposium on In Vitro Culture and Horticultural Breeding
A LARGE SCALE PRODUCTION OF LILIUM BULBLETS THROUGH BIOREACTOR CULTURE |
| Authors: | Y.S. Kim, E.J. Hahn, K.Y. Paek |
| Keywords: | activated charcoal, bioreactor culture, bioreactor type, NH4:NO3 ratio |
| Abstract:
Lilium bulblets were propagated in four types of bioreactor and various environments to determine optimum culture conditions for formation and growth of the bulblets. NH4+: NO3- ratio in the culture medium affected formation and growth of the bulblets. Number of bulblets increased with increasing NH4+-N, showing largest number of bulblets at 3:1 ratio of NH4+ to NO3-. On the other hand, bulblet growth was inhibited at the same NH4+: NO3- ratio (3:1), resulting in the lowest bulblet weight. Leaf and root formation was also severely affected by 3:1 ratio of NH4+ to NO3-. To reduce phenolic compounds the culture medium was circulated through activated charcoal filters. As a result, number of bulblets per scale and total fresh weight significantly increased as compared to control. Morphological features of the bulblets in the medium containing activated charcoal filters were similar to those grown in soil, but a high percentage of abnormal bulblets were observed in the case of not using the activated charcoal filter. In the experiment on bioreactor type, air-lift balloon type favored formation and growth of the bulblets, while stirred jar inhibited bulblet growth. Bulblets in a stirred jar type bioreactor did not survive during the culture period caused by shear stress due to the impeller blades. | |
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