ISHS Acta Horticulturae 560: IV International Symposium on In Vitro Culture and Horticultural Breeding
MOLECULAR TISSUE CULTURE PROCESSING (MTCP): A MOLECULAR AND PHYSIOLOGICAL METHOD TO OPTIMIZE IN VITRO PLANT REGENERATION SYSTEMS IN RECALCITRANT SPECIES |
| Authors: | O. Toldi, P. Scott, M. Truesdale, J. Dettmer, K. Ahanen, S. Sorvari |
| Keywords: | tissue culture, metabolism, photosynthesis, aerobic respiration, DNA methylation, tobacco, carrot |
| Abstract:
The purpose of our study was to investigate how plant growth regulators influence metabolism and development in plant tissue cultures, in order to develop a methodology for forecasting and optimising in vitro morphogenetic processes. The responsive starting explants, as well as the composition of basic compounds and growth regulators of tissue culture media were established through integrated monitoring of methylcytosine/cytosine ratios, efficiencies of photosynthesis, rates of respiration and concentrations of chlorophyll in the plant explants. We call this methodology Molecular Tissue Culture Processing (MTCP). | |
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