ISHS Acta Horticulturae 560: IV International Symposium on In Vitro Culture and Horticultural Breeding
BIOREACTOR TECHNOLOGY IN PLANT CLONING |
| Author: | D.J. Cantliffe |
| Keywords: | synthetic seeds, Ipomoea batatas, somatic embryogenesis, machine vision system, propagation, embryo |
| Abstract:
Plant propagation via somatic embryogenesis has been proposed for synthetic seed production of many crops. Somatic embryos are millimeter-sized plant embryos produced in vitro and have the ability to be genetically cloned, essentially identical to the parent plant. The analysis of bioreactor-based somatic embryogenesis requires the quantification of biological development. This involves the characterization of biological entities in terms of their numbers and distributions according to size and complexity of the cellular structure. Machine-vision is planning an outstanding role in these types of measurements by providing the means to rapidly and objectively quantify plant tissue culture development. The heterogeneity of somatic embryo maturity and quality typically found in embryogenic cultures has impeded the commercialisation of this propagation technique. A machine-vision system was used in our work at the University of Florida to monitor, non-destructively, callus growth during a 10-day period. The machine-vision system was also used to rate the viability of embryos as they were pumped through a 3-mm square glass conduit. Embryo morphologies were ranked and processed by a neuro-network. Solution was run from a bioreactor and objects not harvested were routed back to the bioreactor. The work demonstration the successful in vitro harvest of somatic embryos under non-aseptic conditions when embryos were manually introduced into the harvester. Subsequent work was conducted to improve accuracy of the machine-vision classifier. | |
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