ISHS Acta Horticulturae 560: IV International Symposium on In Vitro Culture and Horticultural Breeding
TRANSFORMATION OF APPLE FOR DURABLE SCAB RESISTANCE: EXPRESSION OF A PUROINDOLINE GENE IN SUSCEPTIBLE AND RESISTANT (Vf) GENOTYPES |
| Authors: | E. Chevreau, F. Dupuis, C. Ortolan, L. Parisi |
| Keywords: | genetic engineering, Malus, puroindoline, Venturia |
| Abstract:
Disease resistance is one of the main objectives of apple (Malus x domestica) breeding. This study aims to compare the effects of the expression of an antifungal transgene into different genetic backgrounds: Galaxy which is scab susceptible, and X6407 which is a recent hybrid carrying the scab resistance gene Vf. Purolindolines are basic cystine-rich proteins belonging to the lipid binding protein group which have been isolated from wheat endosperm. Their antifungal activity has been recently demonstrated. In this study, we have checked in vitro the activity of puroindoline-b against Venturia inaequalis, the causal agent of apple scab. Galaxy and X6407 have been transformed by Agrobacterium tumefaciens EHA105 pPuro-b, containing the puroindoline-b gene under the control of the 35S CaMV promoter, with an efficiency of about 2.5 %. Ten and 11 transgenic clones, respectively, have been produced and checked by PCR analysis. After assessement of their ploidy level by flow cytometry, one tetraploid clone was discarded. Assessment of scab susceptibility of the transgenic clones is now underway. | |
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