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ISHS Acta Horticulturae 560: IV International Symposium on In Vitro Culture and Horticultural Breeding

CHILL-INDUCED REACTIONS IN POPLAR

Authors:   J. Renaut, J.F. Hausman, S. Lutts
Keywords:   Populus tremula x Populus tremuloides, chilling stress, protein, chlorophyll fluorescence
Abstract:
The aim of this work is to study the behaviour of a poplar clone under chilling stress conditions. Two experimental systems constituted of plants or detached leaves of cuttings of Populus tremula x Populus tremuloides cv. Muhs 1 were submitted to chilling (4 °C) and control (23 °C) conditions. During 4 days, sampling was realised. The first step of the work consisted in the observation of the effects of chilling on stress indicators, i.e. chlorophyll fluorescence as well as chlorophyll content. Chlorophyll fluorescence indicated that the plants were stressed. Similarly, increasing sucrose and glucose levels as well as enhanced sucrose metabolism in the plants subjected to chilling stress were recorded. The comparison of the proteome of leaves under both conditions revealed the presence of two families of proteins in stressed plants.

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